Anticorps Polyclonal de lapin anti-B3GAT1

B3GAT1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 19401-1-AP

Synonymes

B3GAT1, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1

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Galerie de données de validation

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  WB analysis using 19401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19401-1-AP (B3GAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 19401-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19401-1-AP (CD57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer de la prostate humain, tissu de cancer de la thyroïde humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

19401-1-AP cible B3GAT1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B3GAT1 Protéine recombinante Ag13550
• Nom complet: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 45 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC010466
• Symbole du gène: B3GAT1
• Identification du gène (NCBI): 27087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 19401-1-AP antibody detectes 45 and 70 kDa forms in SDS-PAGE which might as a result of different degrees of glycosylation or the formation of homodimer.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer.; Authors - Sihui Yu
human	IHC	Gynecol Oncol; Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.; Authors - Jing Zhang
human	IF	Front Bioeng Biotechnol; Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells.; Authors - Jie Chen
human	IHC	BMC Med Genomics; Establishment and validation of an immune infiltration predictive model for ovarian cancer; Authors - Zhenxia Song
human,mouse	IHC	Aging (Albany NY); Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma; Authors - Xiaonan Zhang