Anticorps Polyclonal de lapin anti-Alkyl-DHAP synthase/AGPS

• Phare
• Validé par KD/KO

Alkyl-DHAP synthase/AGPS Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 21011-1-AP

Synonymes

ADAP S, ADAS, ADHAPS, ADPS, AGPS, ALDHPSY, Alkyl DHAP synthase, Alkyl-DHAP synthase/AGPS

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Galerie de données de validation

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  WB analysis of HeLa using 21011-1-AP

  WB result of Alkyl-DHAP synthase/AGPS antibody (21011-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alkyl-DHAP synthase/AGPS transfected HeLa cells.

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  WB analysis using 21011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules SW480, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

21011-1-AP cible Alkyl-DHAP synthase/AGPS dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alkyl-DHAP synthase/AGPS Protéine recombinante Ag15233
• Nom complet: alkylglycerone phosphate synthase
• Masse moléculaire calculée: 658 aa, 73 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC141820
• Symbole du gène: AGPS
• Identification du gène (NCBI): 8540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB	Free Radic Biol Med; NEAT1/hsa-miR-372-3p axis participates in rapamycin-induced lipid metabolic disorder.; Authors - Guanghan Fan ; KD Validated
human	WB	Cell Chem Biol; Context-dependent regulation of ferroptosis sensitivity.; Authors - Leslie Magtanong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (10-28-2022)

Antibody not specific, but AGPS protein was detected in MDA-MB-231 cell line

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7, MDA-MB-231