Anticorps Polyclonal de lapin anti-AZI2/NAP1

AZI2/NAP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15042-1-AP

Synonymes

5 azacytidine induced 2, AZ2, AZI2, AZI2/NAP1, Nak associated protein 1, NAP1, TILP

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Galerie de données de validation

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  WB analysis using 15042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15042-1-AP (AZI2/NAP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15042-1-AP

  IP result of anti-AZI2/NAP1 (IP:15042-1-AP, 4ug; Detection:15042-1-AP 1:300) with HepG2 cells lysate 3000 ug.

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  IP experiment of HEK-293 using 15042-1-AP

  IP result of anti-AZI2/NAP1 (IP:15042-1-AP, 4ug; Detection:15042-1-AP 1:8000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of mouse testis using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15042-1-AP (AZI2/NAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15042-1-AP (AZI2/NAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 15042-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15042-1-AP (AZI2/NAP1 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15042-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AZI2/NAP1 antibody (15042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15042-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AZI2/NAP1 antibody (15042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu testiculaire de souris, tissu d'estomac humain, tissu pancréatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15042-1-AP cible AZI2/NAP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AZI2/NAP1 Protéine recombinante Ag6892
• Nom complet: 5-azacytidine induced 2
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC001139
• Symbole du gène: AZI2
• Identification du gène (NCBI): 64343
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity.

Publications

Species	Application	Title
human	WB	Cancer Res; FIP200 suppresses immune checkpoint therapy responses in breast cancers by limiting AZI2/TBK1/IRF signaling independent of its canonical autophagy function.; Authors - Takako Okamoto
human	WB	PLoS Pathog; MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner.; Authors - Run Fang
human	WB	Front Immunol; Increased IKKϵ protein stability ensures efficient type I interferon responses in conditions of TBK1 deficiency; Authors - Julia Wegner
human	WB,IHC,IF	Autophagy; AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer; Authors - Syn Kok Yeo
human	WB	J Cell Biol; NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis; Authors - Swagatika Paul