Anticorps Polyclonal de lapin anti-Zinc Alpha 2 Glycoprotein

Zinc Alpha 2 Glycoprotein Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13399-1-AP

Synonymes

AZGP1, ZA2G, ZAG, Zinc Alpha 2 Glycoprotein, Zinc-alpha-2-glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1

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Galerie de données de validation

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  WB analysis of human plasma using 13399-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13399-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13399-1-AP (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13399-1-AP (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 13399-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Zinc Alpha 2 Glycoprotein antibody (13399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of PC-3 using 13399-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using Zinc Alpha 2 Glycoprotein antibody (13399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, cellules MCF-7
• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 4 publications below

Informations sur le produit

13399-1-AP cible Zinc Alpha 2 Glycoprotein dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Zinc Alpha 2 Glycoprotein Protéine recombinante Ag4232
• Nom complet: alpha-2-glycoprotein 1, zinc-binding
• Masse moléculaire calculée: 298 aa, 34 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC033830
• Symbole du gène: Zinc Alpha 2 Glycoprotein
• Identification du gène (NCBI): 563
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Publications

Species	Application	Title
human	IHC	Sci Adv; Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.; Authors - Takafumi Kato
human	IHC	Int J Cancer; Reduced AZGP1 expression is an independent predictor of early PSA recurrence and associated with ERG fusion positive and PTEN deleted prostate cancers.; Authors - Christoph Burdelski
human	IHC	J Transl Med; Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis.; Authors - Huang Yan Y
human	WB	Proteome Sci; Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography.; Authors - Lu Chih-Ming CM
human	IHC	Obesity (Silver Spring); PMID: 24753506; Authors - Miroslav Balaz