Anticorps Polyclonal de lapin anti-AUTS2

AUTS2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 25001-1-AP

Synonymes

AUTS2, FBRSL2, KIAA0442

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Galerie de données de validation

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  WB analysis of C6 using 25001-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25001-1-AP (AUTS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25001-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25001-1-AP (AUTS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules C6, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

25001-1-AP cible AUTS2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AUTS2 Protéine recombinante Ag21853
• Nom complet: autism susceptibility candidate 2
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 70 kDa, 130-140 kDa
• Numéro d’acquisition GenBank: BC064693
• Symbole du gène: AUTS2
• Identification du gène (NCBI): 26053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AUTS2 also named as FBRSL2 or autism susceptibility candidate 2 is a 1259 amino acid protein, which belongs to the AUTS2 family. AUTS2 is highly expressed in brain, kidney and skeletal muscle. AUTS2 exists as four isoforms produced by alternative splicing events and is encoded by a gene located on human chromosome 7. The molecular weight of AUTS2 isoform is 65-70 kDa in the brain ( PMID:23332918). The functional role of AUTS2 is not well known, although some studies have identified a putative role in transcriptional regulation during neuronal development.

Publications

Species	Application	Title
human	IF	Front Neurol; Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.; Authors - Jing Huang
human	IF	Neuroscience; PMID: 30599272; Authors - Fangkun Liu
mouse		Mol Cell; Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities.; Authors - Andrea Scelfo
mouse	WB	Exp Neurol; Auts2 regulated autism-like behavior, glucose metabolism and oxidative stress in mice; Authors - Min Liu