Anticorps Polyclonal de lapin anti-AURKA

• Phare
• Validé par KD/KO

AURKA Polyclonal Antibody for IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 10297-1-AP

Synonymes

AIK, ARK 1, ARK1, AURA, AURKA, Aurora A, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

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Galerie de données de validation

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10297-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10297-1-AP (Aurora A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10297-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Aurora A antibody (10297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10297-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Aurora A (10297-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

10297-1-AP cible AURKA dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AURKA Protéine recombinante Ag0362
• Nom complet: aurora kinase A
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC002499
• Symbole du gène: AURKA
• Identification du gène (NCBI): 6790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Publications

Species	Application	Title
human		Acta Pharm Sin B; Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway.; Authors - Xiangjin Zheng
mouse	IF	Nat Commun; CPEB1 directs muscle stem cell activation by reprogramming the translational landscape.; Authors - Wenshu Zeng
human		Oncotarget; Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.; Authors - Yanwei Liu
human		Eur J Med Chem; Design, synthesis, biological evaluation of 6-(2-amino-1H-benzo[d]imidazole-6-yl)quinazolin-4(3H)-one derivatives as novel anticancer agents with Aurora kinase inhibition.; Authors - Chengcheng Fan
human		Front Immunol; The ALDH Family Contributes to Immunocyte Infiltration, Proliferation and Epithelial-Mesenchymal Transformation in Glioma.; Authors - Zeyu Wang
human		J Exp Clin Cancer Res; 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.; Authors - Wan Li