Anticorps Polyclonal de lapin anti-ATXN2L

• Phare
• Validé par KD/KO

ATXN2L Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 24822-1-AP

Synonymes

A2D, A2LG, A2LP, A2RP, Ataxin 2 domain protein, ataxin 2 like, Ataxin 2 like protein, Ataxin 2 related protein, ATXN2L

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Galerie de données de validation

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  WB analysis using 24822-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24822-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24822-1-AP

  IP result of anti-ATXN2L (IP:24822-1-AP, 4ug; Detection:24822-1-AP 1:2000) with HeLa cells lysate 2400ug.

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  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 24822-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ATXN2L antibody (24822-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules MKN-45
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

24822-1-AP cible ATXN2L dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATXN2L Protéine recombinante Ag20581
• Nom complet: ataxin 2-like
• Masse moléculaire calculée: 1062 aa, 112 kDa
• Poids moléculaire observé: ~140 kDa
• Numéro d’acquisition GenBank: BC082760
• Symbole du gène: ATXN2L
• Identification du gène (NCBI): 11273
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.

Publications

Species	Application	Title
human	WB,CoIP	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
mouse	IF,WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
mouse	WB	Int J Mol Sci; Mid-Gestation lethality of Atxn2l-Ablated Mice.; Authors - Jana Key
human	WB	ACS Chem Biol; Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.; Authors - Luam E Araya
rat	WB	Phytother Res; Jatrorrhizine ameliorates Schwann cell myelination via inhibiting HDAC3 ability to recruit Atxn2l for regulating the NRG1-ErbB2-PI3K-AKT pathway in diabetic peripheral neuropathy mice; Authors - Xiaobao Gong
human	WB	Cell Genom; Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders; Authors - Greta Pintacuda

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sahil (Verified Customer) (06-21-2019)

I have checked others too and this works perfectly fine. If needed again would definitely buy it.

• Applications: Western Blot,

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