Anticorps Monoclonal anti-ATX3,ATXN3

ATX3,ATXN3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1F7E10

N° de cat : 67057-1-Ig

Synonymes

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

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Galerie de données de validation

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  WB analysis of MCF-7 using 67057-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67057-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67057-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67057-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67057-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67057-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 67057-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATX3,ATXN3 antibody (67057-1-Ig, Clone: 1F7E10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cérébral de porc, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

67057-1-Ig cible ATX3,ATXN3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATX3,ATXN3 Protéine recombinante Ag6627
• Nom complet: ataxin 3
• Masse moléculaire calculée: 370 aa, 43 kDa
• Poids moléculaire observé: 41-45 kDa
• Numéro d’acquisition GenBank: BC033711
• Symbole du gène: ATXN3
• Identification du gène (NCBI): 4287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

Publications

Species	Application	Title
mouse	IHC	J Clin Invest; CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion; Authors - Shengnan Wang
human	WB	J Biol Chem; ATXN3 functions as a tumor suppresser through potentiating Galectin-9-mediated apoptosis in human colon adenocarcinoma; Authors - Yang Cheng

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