Anticorps Polyclonal de lapin anti-ATX3,ATXN3

• Phare
• Validé par KD/KO

ATX3,ATXN3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 13505-1-AP

Synonymes

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

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Galerie de données de validation

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  WB analysis using 13505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13505-1-AP

  HEK-293 (WT and ATXN3 KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 13505-1-AP incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of mouse heart using 13505-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 13505-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13505-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13505-1-AP

  HEK-293 lysates prepared and IP of ATXN3 performed using 1.0 μg of 13505-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human pancreas cancer using 13505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas cancer using 13505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules HEK-293, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu placentaire de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

13505-1-AP cible ATX3,ATXN3 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris, Caenorhabditis elegans
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATX3,ATXN3 Protéine recombinante Ag4341
• Nom complet: ataxin 3
• Masse moléculaire calculée: 370 aa, 43 kDa
• Poids moléculaire observé: 35-42 kDa
• Numéro d’acquisition GenBank: BC033711
• Symbole du gène: ATXN3
• Identification du gène (NCBI): 4287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

Publications

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu ; KD Validated
human	WB, IF, IP	PLoS Genet; The Role of the Mammalian DNA End-processing Enzyme Polynucleotide Kinase 3'-Phosphatase in Spinocerebellar Ataxia Type 3 Pathogenesis.; Authors - Arpita Chatterjee ; KD Validated
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
human	WB	PLoS Genet; Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3.; Authors - Rui Gao
mouse	WB	Neurobiol Dis; A new humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of neurons and glia and late disease onset of SCA3/MJD.; Authors - Pawel M Switonski