Anticorps Polyclonal de lapin anti-ATP6V1G1

• Phare
• Validé par KD/KO

ATP6V1G1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16143-1-AP

Synonymes

ATP6G, ATP6G1, ATP6GL, ATP6J, ATP6V1G1, V ATPase 13 kDa subunit 1, V ATPase subunit G 1, Vma10

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Galerie de données de validation

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  WB analysis of Jurkat using 16143-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16143-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16143-1-AP

  IP result of anti-ATP6V1G1 (IP:16143-1-AP, 4ug; Detection:16143-1-AP 1:500) with Jurkat cells lysate 3440 ug.

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  IHC staining of human kidney using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16143-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 16143-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16143-1-AP (ATP6V1G1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu rénal humain
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

16143-1-AP cible ATP6V1G1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1G1 Protéine recombinante Ag9217
• Nom complet: ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1
• Masse moléculaire calculée: 118 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC008452
• Symbole du gène: ATP6V1G1
• Identification du gène (NCBI): 9550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Traffic; Fast and cloning-free CRISPR/Cas9-mediated genomic editing in mammalian cells.; Authors - Paul T Manna
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human,mouse	WB,IHC	EBioMedicine; A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.; Authors - Irene Bertolini ; KD Validated
human	WB	EBioMedicine; Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo.; Authors - Andrea Terrasi
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou
mouse	WB	Cell; The proteomic landscape of synaptic diversity across brain regions and cell types; Authors - Marc van Oostrum