Anticorps Polyclonal de lapin anti-ATP5O

ATP5O Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10994-1-AP

Synonymes

ATP5PO, OSCP, ATPO, ATP synthase peripheral stalk subunit OSCP

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Galerie de données de validation

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  WB analysis using 10994-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10994-1-AP (ATP5O antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10994-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10994-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 10994-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10994-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HepG2 using 10994-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ATP5O Polyclonal antibody (10994-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Caco-2, cellules HCT116, cellules HEK-293, cellules HeLa, cellules HepG2, tissu cardiaque de souris, tissu de côlon de souris, tissu hépatique de souris, tissu placentaire de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque humain, tissu de cancer du côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

10994-1-AP cible ATP5O dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP5O Protéine recombinante Ag1458
• Nom complet: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-25 kDa
• Numéro d’acquisition GenBank: BC021233
• Symbole du gène: ATP5O
• Identification du gène (NCBI): 539
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer.; Authors - Robert Wiebringhaus
human	WB	Neural Regen Res; CHCHD2 Thr61Ile mutation impairs F1F0-ATPase assembly in in vitro and in vivo models of Parkinson's disease; Authors - Xiang Chen
human	WB	Brain Pathol; Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.; Authors - Margalida A Frau-Méndez
human	WB	J Mol Biol; COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.; Authors - David Pacheu-Grau
human	WB	Dis Markers; CYC1 Predicts Poor Prognosis in Patients with Breast Cancer.; Authors - Yingyan Han
		Oncol Lett; Lentivirus-mediated silencing of MPHOSPH8 inhibits MTC proliferation and enhances apoptosis.; Authors - Peiyong Li