Anticorps Polyclonal de lapin anti-ATP5H

ATP5H Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (2)

N° de cat : 17589-1-AP

Synonymes

ATP5H, ATP5JD, ATPase subunit d, ATPQ

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Galerie de données de validation

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  WB analysis of Jurkat using 17589-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17589-1-AP (ATP5H antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17589-1-AP

  IP result of anti-ATP5H (IP:17589-1-AP, 3ug; Detection:17589-1-AP 1:2000) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human lung cancer using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17589-1-AP (ATP5H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17589-1-AP (ATP5H Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17589-1-AP (ATP5H Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17589-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17589-1-AP (ATP5H antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cérébral de rat, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

17589-1-AP cible ATP5H dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP5H Protéine recombinante Ag11429
• Nom complet: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
• Masse moléculaire calculée: 137 aa, 16 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC032245
• Symbole du gène: ATP5H
• Identification du gène (NCBI): 10476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.

Publications

Species	Application	Title
human	WB	Brain; Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.; Authors - Xia Feng
mouse	WB	Theranostics; AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.; Authors - Liangjun Xia
human	IHC	Brain Pathol; Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.; Authors - Margalida A Frau-Méndez
human	WB	Oncol Lett; Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX; Authors - Jiang Chang
mouse	WB	J Nanobiotechnology; Modulation of alveolar macrophage and mitochondrial fitness by medicinal plant-derived nanovesicles to mitigate acute lung injury and viral pneumonia; Authors - Lusha Ye
human,mouse	WB	Science; Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists; Authors - Gregory A Wyant

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SITING (Verified Customer) (05-25-2021)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

ATP5H Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:17589-1-AP)

FH

Zee (Verified Customer) (01-28-2020)

It worked well with western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue extract