Anticorps Polyclonal de lapin anti-ATP5F1

ATP5F1 Polyclonal Antibody for WB, IHC, ELISA, IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, CoIP, ELISA, IF/ICC, FC (Intra)

Conjugaison

Non conjugué

Publications

23

Avis (2)

N° de cat : 15999-1-AP

Synonymes

ATP5PB, ATP5F 1, ATP synthase subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase peripheral stalk-membrane subunit b

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Galerie de données de validation

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  WB analysis using 15999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15999-1-AP (ATP5F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15999-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15999-1-AP (ATP5F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15999-1-AP

  Immunofluorescent analysis of Hela cells, using ATP5F1 antibody 15999-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15999-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5F1 (15999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15999-1-AP cible ATP5F1 dans les applications de WB, IHC, CoIP, ELISA, IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP5F1 Protéine recombinante Ag8571
• Nom complet: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
• Masse moléculaire calculée: 256 aa, 29 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC005366
• Symbole du gène: ATP5F1
• Identification du gène (NCBI): 515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

Publications

Species	Application	Title
human	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
rat	WB	Proc Natl Acad Sci U S A; Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation.; Authors - Hidetaka Kioka
mouse	WB,IHC	Part Fibre Toxicol; Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction.; Authors - Quincy A Hathaway
human	WB	Free Radic Biol Med; Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3.; Authors - Qing Zhang
mouse	WB	J Neurosci; A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins.; Authors - Qiu T Ruan
rat	WB	Cell Death Dis; Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.; Authors - Yuehong Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

siting (Verified Customer) (07-13-2020)

very good signal

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse heart cells

ATP5F1 Antibody Western Blot, validation (1:1000 dilution) in mouse heart cells (Cat no:15999-1-AP)

FH

Yan (Verified Customer) (01-28-2020)

It worked very well with mouse heart tissue for western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue extract