Anticorps Polyclonal de lapin anti-ATP5C1

ATP5C1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10910-1-AP

Synonymes

ATP5C, ATP5C1, ATP5CL1, ATPG, F ATPase gamma subunit

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Galerie de données de validation

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  WB analysis using 10910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10910-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 10910-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10910-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10910-1-AP (ATP5C1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); Phalloidin stains F-actin (Red).

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  IF Staining of HepG2 using 10910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10910-1-AP (ATP5C1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque humain, tissu cérébral de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

10910-1-AP cible ATP5C1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP5C1 Protéine recombinante Ag1343
• Nom complet: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC000931
• Symbole du gène: ATP5C1
• Identification du gène (NCBI): 509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB,IP	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
human	WB	J Biol Chem; An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.; Authors - Xiaofen Jin
mouse	WB	PLoS One; Warburg effect's manifestation in aggressive pheochromocytomas and paragangliomas: insights from a mouse cell model applied to human tumor tissue.; Authors - Stephanie M J Fliedner
human	WB	FEBS Lett; Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.; Authors - Makoto Fujikawa
human	WB	iScience; Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis; Authors - Yunfan He