Anticorps Polyclonal de lapin anti-ATG4B

• Phare
• Validé par KD/KO

ATG4B Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 15131-1-AP

Synonymes

APG4B, ATG4B, AUTL1, Autophagin 1, Cysteine protease ATG4B, hAPG4B, KIAA0943

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Galerie de données de validation

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  WB analysis using 15131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15131-1-AP (ATG4B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15131-1-AP

  WB result of ATG4B antibody (15131-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG4B transfected HEK-293 cells.

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  WB analysis using 15131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15131-1-AP (ATG4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15131-1-AP (ATG4B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15131-1-AP

  IP result of anti-ATG4B (IP:15131-1-AP, 4ug; Detection:15131-1-AP 1:800) with HeLa cells lysate 1560 ug.

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  IHC staining of human pancreas cancer using 15131-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15131-1-AP (ATG4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15131-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15131-1-AP (ATG4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15131-1-AP (ATG4B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 15131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15131-1-AP (ATG4B antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 22 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

15131-1-AP cible ATG4B dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG4B Protéine recombinante Ag7261
• Nom complet: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC000719
• Symbole du gène: ATG4B
• Identification du gène (NCBI): 23192
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.

Publications

Species	Application	Title
human	WB,IF,IP	Autophagy; ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1; Authors - Weihong Xie ; KO Validated
rat	WB	Autophagy; RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.; Authors - Luis Muniz-Feliciano
human	WB,IF	Free Radic Biol Med; The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.; Authors - Zhiguo Li ; KD Validated
human	WB	Cell Biosci; Discovery and mechanism studies of a novel ATG4B inhibitor Ebselen by drug repurposing and its anti-colorectal cancer effects in mice; Authors - Huazhong Xie ; KO Validated
human	WB	Cancer Lett; HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells.; Authors - Nan Zhang
human	WB	Front Cell Dev Biol; LncRNA CRNDE Promotes ATG4B-Mediated Autophagy and Alleviates the Sensitivity of Sorafenib in Hepatocellular Carcinoma Cells.; Authors - Lingxi Chen ; KD Validated