Anticorps Polyclonal de lapin anti-ASPH

• Phare
• Validé par KD/KO

ASPH Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14116-1-AP

Synonymes

AAH, ASP beta hydroxylase, aspartate beta hydroxylase, ASPH, BAH, CASQ2BP1, HAAH, JCTN, junctin

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Galerie de données de validation

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  WB analysis of A549 using 14116-1-AP

  WB result of ASPH antibody (14116-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASPH transfected A549 cells.

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  WB analysis of mouse brain using 14116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14116-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14116-1-AP (ASPH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14116-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14116-1-AP (ASPH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14116-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14116-1-AP (ASPH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, tissu cérébral humain
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

14116-1-AP cible ASPH dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASPH Protéine recombinante Ag5265
• Nom complet: aspartate beta-hydroxylase
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC066929
• Symbole du gène: ASPH
• Identification du gène (NCBI): 444
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aspartate-β-hydroxylase (ASPH; termed AAH in older literature) has been implicated in the cross-talk among all of these signaling pathways. Correspondingly, ASPH is expressed at high levels in many malignant neoplasms of different histogeneses. It has the 86-141 kDa bands (native and phosphorylated forms), cleavage products of 35-56 and 22-26 kDa.(PMID:27981247/PMID: 10956665)

Publications

Species	Application	Title
human	WB	EBioMedicine; Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway.; Authors - Qifei Zou ; KD Validated
human	WB	J Immunol Res; Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma.; Authors - Yujiao Zhou
human,rat	WB,IHC	Heliyon; Labyrinthin: A distinct pan-adenocarcinoma diagnostic and immunotherapeutic tumor specific antigen.; Authors - Michael Babich
human	WB	Biochem Pharmacol; Inhibition of circular RNA ASPH reduces the proliferation and promotes the apoptosis of hepatic stellate cells in hepatic fibrosis; Authors - Hongwu Meng