Anticorps Polyclonal de lapin anti-ASL

ASL Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16645-1-AP

Synonymes

argininosuccinate lyase, Arginosuccinase, ASAL, ASL

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Galerie de données de validation

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  WB analysis using 16645-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16645-1-AP (ASL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16645-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16645-1-AP (ASL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16645-1-AP (ASL Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 16645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16645-1-AP (ASL Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

16645-1-AP cible ASL dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASL Protéine recombinante Ag10007
• Nom complet: argininosuccinate lyase
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC008195
• Symbole du gène: ASL
• Identification du gène (NCBI): 435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)

Publications

Species	Application	Title
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
human,mouse	WB	Cell Discov; TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential; Authors - Jing He
mouse	WB	Sci Transl Med; Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin; Authors - PamelaSara E Head
human	WB,IHC	J Proteome Res; Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.; Authors - Yin Cao