Anticorps Polyclonal de lapin anti-ASH2L

• Phare
• Validé par KD/KO

ASH2L Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe, souris

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12331-1-AP

Synonymes

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

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Galerie de données de validation

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  WB analysis of COS-7 using 12331-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12331-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12331-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12331-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12331-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 12331-1-AP

  IP result of anti-ASH2L (IP:12331-1-AP, 4ug; Detection:12331-1-AP 1:500) with COLO 320 cells lysate 2000ug.

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  IHC staining of human gliomas using 12331-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12331-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COS-7, cellules COLO 320
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

12331-1-AP cible ASH2L dans les applications de WB, IP, IF, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASH2L Protéine recombinante Ag3008
• Nom complet: ash2 (absent, small, or homeotic)-like (Drosophila)
• Masse moléculaire calculée: 628 aa, 69 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC015936
• Symbole du gène: ASH2L
• Identification du gène (NCBI): 9070
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.; Authors - Esteban A Orellana
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB,IHC,IF,IP,ChIP	Cancer Sci; ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription.; Authors - Kai Zeng ; KD Validated
mouse	WB,IP	Mol Neurobiol; WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling.; Authors - Minhan Ka