Anticorps Polyclonal de lapin anti-ASF1A

ASF1A Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 10784-1-AP

Synonymes

ASF1A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146

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Galerie de données de validation

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  WB analysis of HeLa using 10784-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10784-1-AP (ASF1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10784-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10784-1-AP (ASF1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10784-1-AP

  IP result of anti-ASF1A (IP:10784-1-AP, 4ug; Detection:10784-1-AP 1:800) with HEK-293 cells lysate 2800ug.

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  IHC staining of human lymphoma using 10784-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10784-1-AP (ASF1A antibody) at dilution of 1:10 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

10784-1-AP cible ASF1A dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASF1A Protéine recombinante Ag1233
• Nom complet: ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 204 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC010878
• Symbole du gène: ASF1A
• Identification du gène (NCBI): 25842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Publications

Species	Application	Title
human	WB,IF,IP	J Virol; Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3.; Authors - Ambagala Aruna P AP
mouse	WB,IF	Epigenetics Chromatin; Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice.; Authors - Xuemei Wang
human	IF	PLoS One; HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.; Authors - Jiang Wei-Qin WQ
human	IHC,WB	Sci Rep; Identification of ASF1A and HJURP by global H3-H4 histone chaperone analysis as a prognostic two-gene model in hepatocellular carcinoma; Authors - Yongkang Liu
human	IHC	Front Pharmacol; Prognostic characteristics and drug sensitivity analysis of hepatocellular carcinoma based on histone modification-related genes: a multi-omics integrated study revealing potential therapeutic targets and individualized treatment strategies; Authors - Ping Sun
human	WB	Biol Proced Online; A novel telomere-associated genes signature for the prediction of prognosis and treatment responsiveness of hepatocellular carcinoma; Authors - Kuo Kang

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