Anticorps Polyclonal de lapin anti-ASCC3

ASCC3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 17627-1-AP

Synonymes

ASC 1 complex subunit p200, ASC1p200, ASCC3, HELIC1, Helicase, ATP binding 1, RNAH, Trip4 complex subunit p200



Applications testées

Résultats positifs en WBcellules HeLa, cellules HepG2
Résultats positifs en IPcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

17627-1-AP cible ASCC3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ASCC3 Protéine recombinante Ag11848
Nom complet activating signal cointegrator 1 complex subunit 3
Masse moléculaire calculée 111 aa, 13 kDa, 251 kDa
Poids moléculaire observé 251 kDa
Numéro d’acquisition GenBankBC050681
Symbole du gène ASCC3
Identification du gène (NCBI) 10973
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Protocole

Product Specific Protocols
WB protocol for ASCC3 antibody 17627-1-APDownload protocol
IP protocol for ASCC3 antibody 17627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP,WB

Nat Commun

Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex

Authors - Junqiao Jia
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
humanWB

Mol Cell

Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.

Authors - Mark Stoneley
humanWB

Sci Rep

Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells.

Authors - Satoshi Hashimoto
mouseWB

Cell Metab

Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response

Authors - Goda Snieckute

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (10-24-2023)

(Already in contact with technical support, but was asked to submit a review here as well.) Immunoblotting for ASCC3 from fresh cell extracts did not work. Included samples of ASCC3-KD (siRNA) experiment, but only unspecific bands. Tested 1:1000 and 1:500 dilution. Tested different protein extraction buffers and conditions, used fresh cells each time. Transfer of large proteins was confirmed. Sadly, for us this antibody did not work.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 and 1:500
  • Cell Tissue Type: HEK293