Anticorps Polyclonal de lapin anti-ASCC3

ASCC3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 17627-1-AP

Synonymes

ASC 1 complex subunit p200, ASC1p200, ASCC3, HELIC1, Helicase, ATP binding 1, RNAH, Trip4 complex subunit p200

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Galerie de données de validation

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  WB analysis of HeLa using 17627-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17627-1-AP (ASCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17627-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17627-1-AP (ASCC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17627-1-AP

  IP result of anti-ASCC3 (IP:17627-1-AP, 4ug; Detection:17627-1-AP 1:400) with HeLa cells lysate 1080ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

17627-1-AP cible ASCC3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASCC3 Protéine recombinante Ag11848
• Nom complet: activating signal cointegrator 1 complex subunit 3
• Masse moléculaire calculée: 111 aa, 13 kDa, 251 kDa
• Poids moléculaire observé: 251 kDa
• Numéro d’acquisition GenBank: BC050681
• Symbole du gène: ASCC3
• Identification du gène (NCBI): 10973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IP,WB	Nat Commun; Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex; Authors - Junqiao Jia
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human	WB	Mol Cell; Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.; Authors - Mark Stoneley
human	WB	Sci Rep; Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells.; Authors - Satoshi Hashimoto
mouse	WB	Cell Metab; Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response; Authors - Goda Snieckute

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (10-24-2023)

(Already in contact with technical support, but was asked to submit a review here as well.) Immunoblotting for ASCC3 from fresh cell extracts did not work. Included samples of ASCC3-KD (siRNA) experiment, but only unspecific bands. Tested 1:1000 and 1:500 dilution. Tested different protein extraction buffers and conditions, used fresh cells each time. Transfer of large proteins was confirmed. Sadly, for us this antibody did not work.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 and 1:500
• Cell Tissue Type: HEK293