Anticorps Polyclonal de lapin anti-ASB5

ASB5 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 24031-1-AP

Synonymes

ASB 5, ASB5

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Galerie de données de validation

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  WB analysis using 24031-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 24031-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 24031-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 24031-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 24031-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASB5 antibody (24031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de souris, tissu de muscle squelettique de rat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

24031-1-AP cible ASB5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASB5 Protéine recombinante Ag21230
• Nom complet: ankyrin repeat and SOCS box-containing 5
• Masse moléculaire calculée: 329 aa, 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC065710
• Symbole du gène: ASB5
• Identification du gène (NCBI): 140458
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ASB5, also named as Ankyrin repeat and SOCS box-containing 5, is a 329 amino acid protein, which contains a conserved one C-terminal SOCS box motif. Asb5 was significantly upregulated in growing collateral arteries on mRNA and protein level. It has been shown that asb5 is a protein implicated in the initiation of arteriogenesis.