Anticorps Polyclonal de lapin anti-Arrestin C

Arrestin C Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 11100-2-AP

Synonymes

ARR3, Arrestin C, ARRX, C arrestin, CAR, cArr, Cone arrestin, Retinal cone arrestin 3, X arrestin

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Galerie de données de validation

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  WB analysis of human brain using 11100-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11100-2-AP (Arrestin C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 11100-2-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 11100-2-AP (Arrestin C antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11100-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11100-2-AP (Arrestin C antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 11100-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11100-2-AP (Arrestin C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 11100-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11100-2-AP (Arrestin C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 11100-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Arrestin C antibody (11100-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 11100-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Arrestin C antibody (11100-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 11100-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Arrestin C antibody (11100-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Retinal organoids using 11100-2-AP

  Retinal organoids (day 220) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA, Stained for Arrestin C with 11100-2-AP at 1:400. Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cardiaque de souris, tissu oculaire de souris
• Résultats positifs en IHC: tissu oculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: Retinal organoids, tissu oculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 5 publications below

Informations sur le produit

11100-2-AP cible Arrestin C dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Arrestin C Protéine recombinante Ag1580
• Nom complet: arrestin 3, retinal (X-arrestin)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC012096
• Symbole du gène: Arrestin C
• Identification du gène (NCBI): 407
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Nat Commun; A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression.; Authors - Jing Liu
human	IHC	Cancers (Basel); Clinical, Genomic, and Pharmacological Study of MYCN-Amplified RB1 Wild-Type Metastatic Retinoblastoma.; Authors - Santiago Zugbi
human	IHC	Int J Mol Sci; Tridimensional Retinoblastoma Cultures as Vitreous Seeds Models for Live-Cell Imaging of Chemotherapy Penetration.; Authors - Ursula Winter
mouse	WB	Front Mol Neurosci; A mouse model of cone photoreceptor function loss (cpfl9) with degeneration due to a mutation in Gucy2e; Authors - Anna S E N Naggert
human	IHC	Pediatr Blood Cancer; Immunohistochemical expression of TFF1 is a marker of poor prognosis in retinoblastoma; Authors - Rosario Aschero
human	IHC	Invest Ophthalmol Vis Sci; Establishment and Comprehensive Characterization of a Novel Preclinical Platform of Metastatic Retinoblastoma for Therapeutic Developments; Authors - Santiago Zugbi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (01-19-2023)

great outcome, no unspecific staining

• Applications: Immunofluorescence