Anticorps Polyclonal de lapin anti-ARPC5

ARPC5 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16717-1-AP

Synonymes

ARC16, Arp2/3 complex 16 kDa subunit, ARPC5, p16 Arc

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Galerie de données de validation

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  WB analysis of mouse brain using 16717-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 16717-1-AP

  Rat brain lysates were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16717-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16717-1-AP (ARPC5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 16717-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ARPC5 antibody (16717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de thymus de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

16717-1-AP cible ARPC5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARPC5 Protéine recombinante Ag10144
• Nom complet: actin related protein 2/3 complex, subunit 5, 16kDa
• Masse moléculaire calculée: 154 aa, 17 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC057237
• Symbole du gène: ARPC5
• Identification du gène (NCBI): 10092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis; Authors - Jiying Hou
human	WB	J Neurosci; The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse.; Authors - Avanti Gokhale
human	WB	Pigment Cell Melanoma Res; YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5); Authors - Jason W. Lui
human	IHC	BMC Cancer; ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas; Authors - Yue Ming
human	WB	Heliyon; Dapagliflozin impedes endothelial cell senescence by activating the SIRT1 signaling pathway in type 2 diabetes; Authors - Shi Tai