Anticorps Polyclonal de lapin anti-ARPC4

ARPC4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10930-1-AP

Synonymes

ARC20, Arp2/3 complex 20 kDa subunit, ARPC4, p20 Arc

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Galerie de données de validation

• 

  WB analysis of mouse brain using 10930-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10930-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10930-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of 4T1 using 10930-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse small intestine using 10930-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 10930-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lymph using 10930-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10930-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10930-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules 4T1, cellules MCF-7, tissu cérébral humain, tissu de cervelet de souris, tissu d'intestin grêle de souris, tissu lymphatique de rat, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain, tissu de carcinome urothélial humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

10930-1-AP cible ARPC4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARPC4 Protéine recombinante Ag1366
• Nom complet: actin related protein 2/3 complex, subunit 4, 20kDa
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 19-25 kDa
• Numéro d’acquisition GenBank: BC012596
• Symbole du gène: ARPC4
• Identification du gène (NCBI): 10093
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ARP2/3 complex consists of seven subunits: two actin-related proteins (ARP2 and ARP3) and five ARP2/3 complex polypeptides (ARPC1B and ARPC2-5). ARPC4 is a core subunit of the actin-related protein (ARP2/3) complex, which catalyzes the formation of F-actin networks. ARPC4 is essential for the structural integrity of the ARP2/3 complex function. Mutations in ARPC4 cause Developmental delay, language impairment, and ocular abnormalities (DEVLO)(PMID: 35047857).

Publications

Species	Application	Title
human	WB	Life Sci Alliance; A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells; Authors - Sumana Sharma
human	WB	Biochim Biophys Acta Proteins Proteom; Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis.; Authors - Guoxuan Qin
human	WB	Drug Resist Updat; Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane; Authors - Xin Zhang