Anticorps Polyclonal de lapin anti-ARNT2

• Phare
• Validé par KD/KO

ARNT2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 12810-1-AP

Synonymes

ARNT protein 2, ARNT2, bHLHe1, KIAA0307

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Galerie de données de validation

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  WB analysis of Jurkat using 12810-1-AP

  WB result of ARNT2 antibody (12810-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT2 transfected Jurkat cells.

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  WB analysis of HEK-293 using 12810-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12810-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12810-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 12810-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12810-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12810-1-AP (ARNT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12810-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12810-1-AP (ARNT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules Raji
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12810-1-AP cible ARNT2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARNT2 Protéine recombinante Ag3536
• Nom complet: aryl-hydrocarbon receptor nuclear translocator 2
• Masse moléculaire calculée: 716 aa, 79 kDa
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC036099
• Symbole du gène: ARNT2
• Identification du gène (NCBI): 9915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aryl-hydrocarbon receptor nuclear translocator 2(ARNT2), is a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. It specifically recognizes the xenobiotic response element(XRE). ARNT2 plays an important role in normal glucose handling in pancreatic beta cell function in humans and mice. ARNT2 proteins are dimeric partners for other PAS proteins such as HIF and SIM. ARNT2 and the other ARNT family members, e.g., ARNT, hypoxin inducible factor 1α (HIF1α), HIF2α, and aryl hydrocarbon receptor (AhR), are thought to function as heterodimers, and regulate the expression, and thereby the function, of many genes. Under hypoxic conditions, it complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. Modulation of ARNT2 in pancreatic beta cells affects glucose stimulated INS secretion (GSIS). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human ARNT2.

Publications

Species	Application	Title
mouse	WB	Front Microbiol; Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection.; Authors - Zhixiao Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristina (Verified Customer) (12-16-2019)

Works well for WB analysis on MDA-MB-231 and 293T cells. Very clean.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mammalian cells