Anticorps Monoclonal anti-ARL8A/ARL8B

ARL8A/ARL8B Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2F4C3

N° de cat : 68186-1-Ig

Synonymes

ARL10B, ARL8A, FLJ45195, GIE2

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Galerie de données de validation

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  WB analysis using 68186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 68186-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de lapin, cellules HeLa, cellules LNCaP, tissu cérébral de rat, tissu cérébral de souris, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68186-1-Ig cible ARL8A/ARL8B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARL8A/ARL8B Protéine recombinante Ag10758
• Nom complet: ADP-ribosylation factor-like 8A
• Masse moléculaire calculée: 186 aa, 21 kDa
• Poids moléculaire observé: 18 kDa, 21 kDa
• Numéro d’acquisition GenBank: BC015408
• Symbole du gène: ARL8A
• Identification du gène (NCBI): 127829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A/ARL8B can be recognized by this antibody.