Anticorps Polyclonal de lapin anti-ARL3

• Phare
• Validé par KD/KO

ARL3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 10961-1-AP

Synonymes

ADP ribosylation factor like 3, ARFL3, ARL3

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Galerie de données de validation

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  WB analysis using 10961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10961-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10961-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10961-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10961-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10961-1-AP (ARL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10961-1-AP (ARL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of IMCD3 cells using 10961-1-AP

  IF result of 10961-1-AP (anti-ARL3,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus).

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  IF Staining of hTERT-RPE1 using 10961-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 10961-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules PC-3, tissu cérébral de rat, tissu cérébral humain, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MDCK, cellules hTERT-RPE1, cellules IMCD3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

10961-1-AP cible ARL3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARL3 Protéine recombinante Ag1406
• Nom complet: ADP-ribosylation factor-like 3
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20 kDa, 25 kDa
• Numéro d’acquisition GenBank: BC009841
• Symbole du gène: ARL3
• Identification du gène (NCBI): 403
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	WB,IF	Nat Commun; Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation.; Authors - Antonios D Konitsiotis
mouse	WB,IF, IP	Nat Commun; Arl3 and LC8 regulate dissociation of dynactin from dynein.; Authors - Mingyue Jin ; KD Validated
mouse	WB	Nat Commun; Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.; Authors - Hyunho Kim ; KD Validated
human	WB	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC
human	IF	Dev Cell; The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK.; Authors - Louise A Stephen