Anticorps Polyclonal de lapin anti-ARHGEF7

ARHGEF7 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 14092-1-AP

Synonymes

ARHGEF7, BETA PIX, COOL 1, COOL1, KIAA0142, KIAA0412, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PAK3BP, PIXB

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Galerie de données de validation

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  WB analysis of PC-12 using 14092-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14092-1-AP

  WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negatove control and ARHGEF7 overexpressed Transfected HEK-293 cells.

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  WB analysis of Jurkat using 14092-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14092-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14092-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14092-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14092-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14092-1-AP

  IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 14092-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules HEK-293 transfectées, cellules HeLa, cellules Jurkat, cellules K-562, cellules NIH/3T3, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

14092-1-AP cible ARHGEF7 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARHGEF7 Protéine recombinante Ag5223
• Nom complet: Rho guanine nucleotide exchange factor (GEF) 7
• Masse moléculaire calculée: 803 aa, 90 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC060776
• Symbole du gène: ARHGEF7
• Identification du gène (NCBI): 8874
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7.; Authors - Ruth Chia
human	WB,IHC	Am J Transl Res; Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.; Authors - Tao Ma