Anticorps Polyclonal de lapin anti-ARHGDIB

• Phare
• Validé par KD/KO

ARHGDIB Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16122-1-AP

Synonymes

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

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Galerie de données de validation

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  WB analysis using 16122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 16122-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16122-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 16122-1-AP

  IP result of anti-ARHGDIB (IP:16122-1-AP, 4ug; Detection:16122-1-AP 1:1000) with Raji cells lysate 2000ug.

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  IHC staining of human stomach cancer using 16122-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16122-1-AP (ARHGDIB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 16122-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16122-1-AP (ARHGDIB antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Jurkat, cellules K-562, cellules Raji, tissu splénique de rat
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

16122-1-AP cible ARHGDIB dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARHGDIB Protéine recombinante Ag9078
• Nom complet: Rho GDP dissociation inhibitor (GDI) beta
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC009200
• Symbole du gène: ARHGDIB
• Identification du gène (NCBI): 397
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Publications

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Nat Immunol; Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.; Authors - Jing Geng
human	WB	Mol Cancer; KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1.; Authors - Lei Liu
human	WB	Proteomics; Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.; Authors - Fan Zhang
human	WB,IHC	Cancer Biomark; Systematic investigation of biomarker-like role of ARHGDIB in breast cancer.; Authors - Xiaonan Wang ; KD Validated
human	WB	DNA Cell Biol; Rho GDP Dissociation Inhibitor (GDI) Beta Promotes Cell Proliferation and Invasion by Modulating the AKT Pathway in Hepatocellular Carcinoma.; Authors - Yang Fang