Anticorps Polyclonal de lapin anti-RhoGDI

• Phare
• Validé par KD/KO

RhoGDI Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 10509-1-Ig

Synonymes

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1

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Galerie de données de validation

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  WB analysis of Jurkat using 10509-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10509-1-Ig (RhoGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10509-1-Ig

  WB result of RhoGDI antibody (10509-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells.

• 

  WB analysis of HeLa using 10509-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 10509-1-Ig (RhoGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10509-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10509-1-Ig (RhoGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10509-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 10509-1-Ig (RhoGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 10509-1-Ig

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10509-1-Ig (RhoGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10509-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10509-1-Ig (RhoGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10509-1-Ig(RhoGDI antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10509-1-Ig(RhoGDI antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10509-1-Ig(RhoGDI antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10509-1-Ig(RhoGDI antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules HEK-293, cellules HeLa, cellules HL-60, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 1 publications below

Informations sur le produit

10509-1-Ig cible RhoGDI dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RhoGDI Protéine recombinante Ag0791
• Nom complet: Rho GDP dissociation inhibitor (GDI) alpha
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC005851
• Symbole du gène: RhoGDI
• Identification du gène (NCBI): 396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.

Publications

Species	Application	Title
human	WB	Cancer Res; miR-483-5p Promotes Invasion and Metastasis of Lung Adenocarcinoma by Targeting RhoGDI1 and ALCAM.; Authors - Qiancheng Song ; KD Validated
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
human	WB	J Proteome Res; Quantitative analysis of the detergent-insoluble brain proteome in frontotemporal lobar degeneration using SILAC internal standards.; Authors - Seyfried Nicholas T NT
human	WB	J Exp Clin Cancer Res; Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.; Authors - Yu Lian
human	WB	PLoS One; Comparative expression profiling of distinct T cell subsets undergoing oxidative stress.; Authors - Lichtenfels Rudolf R

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (11-26-2023)

Worked well at 1:2000 for Glioblastoma cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: GBM