Anticorps Polyclonal de lapin anti-GRAF

GRAF Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 17747-1-AP

Synonymes

ARHGAP26, GRAF, KIAA0621, Oligophrenin 1 like protein, OPHN1L, OPHN1L1

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Galerie de données de validation

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  WB analysis of HeLa using 17747-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17747-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17747-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17747-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17747-1-AP

  IP result of anti-GRAF (IP:17747-1-AP, 4ug; Detection:17747-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of human liver using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu cérébral humain, tissu de thymus de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17747-1-AP cible GRAF dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRAF Protéine recombinante Ag12008
• Nom complet: Rho GTPase activating protein 26
• Masse moléculaire calculée: 759 aa, 86 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC068555
• Symbole du gène: ARHGAP26
• Identification du gène (NCBI): 23092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GTPase Regulator Associated with Focal Adhesion Kinase (GRAF), also known as Rho GTPase activating protein 26 (ARHGAP26) , is a GTPase-activating protein and inhibits the activity of Rho GTPases by promoting the hydrolytic ability of Rho GTPases. GRAF enhances the hydrolysis of GTPases and converts GTPases from an active form to an inactive form, thereby inhibiting signaling transduction. Deletion and mutation of GRAF can lead to promyelocytic leukemia, suggesting tumor suppressive activity of GRAF. GRAF was downregulated in glioblastoma and associated with cell proliferation and migration (PMID: 10908648, PMID: 17611651, PMID: 31004081). It has been reported that there are three splicing mutants of GRAF, namely GRAF1a (92 kDa),GRAF1b (86 kDa) and GRAF1c (75-82 kDa), and GRAF1b and GRAF1c are the major GRAF1 isoforms in adult brain, whereas GRAF1a is abundant in neonates(PMID: 35624318, PMID: 30626696).

Publications

Species	Application	Title
mouse	WB	J Neurol; Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis; Authors - Sven Jarius
human	WB	Brain Res; Deficiency of NEAT1 prevented MPP+-induced inflammatory response, oxidative stress and apoptosis in dopaminergic SK-N-SH neuroblastoma cells via miR-1277-5p/ARHGAP26 axis.; Authors - Shufang Zhou
human	WB,IHC,CoIP	Cell Death Dis; CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway; Authors - Guojie Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cicek (Verified Customer) (06-11-2024)

I used this antibody on mouse neurons, iPSCs and iNeurons with Western blotting, using beta-actin as positive control. There were specific bands at 90 kDa mark for iPSCs and iNeurons, but not for mouse neurons. It was highly expressed in iNeurons. There were unspecific bands at the 70 kDa mark for all samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000