Anticorps Polyclonal de lapin anti-ARHGAP24

ARHGAP24 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 18834-1-AP

Synonymes

ARHGAP24, Filamin A associated RhoGAP, FILGAP, p73, p73RhoGAP, RC GAP72, RCGAP72, Rho type GTPase activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY SAR 88

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Galerie de données de validation

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  IHC staining of human kidney using 18834-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18834-1-AP (ARHGAP24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18834-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18834-1-AP (ARHGAP24 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

18834-1-AP cible ARHGAP24 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Rho GTPase activating protein 24
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC098580
• Symbole du gène: ARHGAP24
• Identification du gène (NCBI): 83478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARHGAP24, also named as FILGAP, is involved in cell polarity, cell morphology and cytoskeletal organization. ARHGAP24 acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. ARHGAP24 controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. ARHGAP24 can suppress the activity of RAC1 and CDC42 in vitro. Its overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. The antibody may recognize all the isoforms of ARHGAP24 except isoform 5.

Publications

Species	Application	Title
human	IF	Curr Biol; Cyclic Mechanical Loading Is Essential for Rac1-Mediated Elongation and Remodeling of the Embryonic Mitral Valve.; Authors - Russell A Gould
human	WB,IHC	Oncogene; Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.; Authors - Xin Jin
human	IHC	Oncotarget; Global gene expression in pseudomyxoma peritonei, with parallel development of two immortalized cell lines.; Authors - Darren L Roberts
human	WB	Clin Exp Pharmacol Physiol; ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42.; Authors - Li Wang
human,mouse	WB,IF	Biochem Biophys Rep; Knockdown of ARHGAP24 reduces intimal hyperplasia through inhibiting the proliferation and phenotypic switching of smooth muscle cells possibly by inactivating both AKT and ERK1/2 signaling pathways; Authors - Wei Liu