Anticorps Polyclonal de lapin anti-ARHGAP21

• Phare
• Validé par KD/KO

ARHGAP21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 22183-1-AP

Synonymes

Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10

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Galerie de données de validation

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  WB analysis using 22183-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 22183-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 22183-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 22183-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 22183-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 22183-1-AP

  Immunofluorescent analysis of HepG2 cells using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3
• Résultats positifs en IHC: tissu ovarien humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

22183-1-AP cible ARHGAP21 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARHGAP21 Protéine recombinante Ag16515
• Nom complet: Rho GTPase activating protein 21
• Masse moléculaire calculée: 1957 aa, 217 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC118915
• Symbole du gène: ARHGAP21
• Identification du gène (NCBI): 57584
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Sci Adv; Phosphorylation-dependent recognition of diverse protein targets by the cryptic GK domain of MAGI MAGUKs; Authors - Meng Zhang ; KD Validated
human	WB,IF	Elife; PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.; Authors - Arman Javadi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

first 2 lanes are whole cell lysate. ARHGAP21doesn't show up prominently but does show up in last 2 lanes which are purified biotinylated proteins surrounding out protein of interest. This seems to indicate that the antibody works but just that the cells we use express levels that are below the level of detection unless it is enriched (such as with our purification protocol)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

ARHGAP21 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22183-1-AP)