Anticorps Polyclonal de lapin anti-ARFGAP3

• Phare
• Validé par KD/KO

ARFGAP3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15293-1-AP

Synonymes

ARF GAP 3, ARFGAP1, ARFGAP3

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Galerie de données de validation

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  WB analysis of HepG2 using 15293-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15293-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15293-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15293-1-AP

  IP result of anti-ARFGAP3 (IP:15293-1-AP, 4ug; Detection:15293-1-AP 1:2000) with HepG2 cells lysate 3000 ug.

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  IHC staining of human breast cancer using 15293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 15293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15293-1-AP

  Immunofluorescent analysis of A431 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of A431 using 15293-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of A431 using 15293-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of HepG2 using 15293-1-AP

  Immunofluorescent analysis of HepG2 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

15293-1-AP cible ARFGAP3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARFGAP3 Protéine recombinante Ag7536
• Nom complet: ADP-ribosylation factor GTPase activating protein 3
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-66 kDa
• Numéro d’acquisition GenBank: BC005122
• Symbole du gène: ARFGAP3
• Identification du gène (NCBI): 26286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARFGAP3, also named as ADP-ribosylation factor GTPase-activating protein 3, is a 516 amino acid protein, which is Widely expressed with Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate). ARFGAP3 is a GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. The calculated molecular weight of ARFGAP3 is 57 kDa, but modified ARFGAP3 is about 57-66 kDa.

Publications

Species	Application	Title
human	WB	BMC Biol; ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.; Authors - Xihua Yue ; KD Validated
human	WB	Commun Biol; Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.; Authors - Xihua Yue
mouse	IF	Cell Signal; ArfGAP3 regulates vesicle transport and glucose uptake in myoblasts; Authors - Suting Li