Anticorps Polyclonal de lapin anti-ARFGAP1

• Phare
• Validé par KD/KO

ARFGAP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 13571-1-AP

Synonymes

ARF GAP 1, ARF1 GAP, ARF1GAP, ARFGAP1, HRIHFB2281

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Galerie de données de validation

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  WB analysis of PC-3 using 13571-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13571-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13571-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13571-1-AP

  IP result of anti-ARFGAP1 (IP:13571-1-AP, 4ug; Detection:13571-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 13571-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 13571-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 13571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa, tissu cérébral humain
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

13571-1-AP cible ARFGAP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARFGAP1 Protéine recombinante Ag4468
• Nom complet: ADP-ribosylation factor GTPase activating protein 1
• Masse moléculaire calculée: 414 aa, 46 kDa
• Poids moléculaire observé: 46 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC028233
• Symbole du gène: ARFGAP1
• Identification du gène (NCBI): 55738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles. The assembly-disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP-GDP cycle of the small G protein ARF1. ARFGAP1 contributes to vesicle budding by increasing the GTPase activity of ARF1, because it promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. for ARFGAP1 may has a role in regulating the GTPase activity and neuronal toxicity of LRRK2; reciprocally, LRRK2 phosphorylates ArfGAP1 and is required for ArfGAP1 neuronal toxicity.

Publications

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
mouse,rat	WB,IF	PLoS Genet; GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.; Authors - Klodjan Stafa ; KD Validated
human	WB, IF	FASEB J; ArfGAP1 acts as a GTPase-activating protein for human ADP-ribosylation factor-like 1 protein.; Authors - Hsiang-Pu Feng ; KD Validated
human,pig	WB	Vet Microbiol; ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.; Authors - Liang Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (10-27-2021)

This antibody clearly label the Golgi localisation of endogenous ArfGAP1, and also gives a clean signal in WB.

• Applications: Western Blot, Immunofluorescence

FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

ARFGAP1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:13571-1-AP)