Anticorps Polyclonal de lapin anti-ARF6-Specific

• Phare
• Validé par KD/KO

ARF6-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 20225-1-AP

Synonymes

ADP ribosylation factor 6, ARF6, ARF6-Specific

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Galerie de données de validation

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  WB analysis using 20225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20225-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20225-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 20225-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20225-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20225-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20225-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules HEK-293, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu de côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Informations sur le produit

20225-1-AP cible ARF6-Specific dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ADP-ribosylation factor 6
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: NM_001663
• Symbole du gène: ARF6
• Identification du gène (NCBI): 382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
human	WB	Int J Biol Sci; Platelet-derived extracellular vesicles aggravate septic acute kidney injury via delivering ARF6; Authors - Xun Lu ; KD Validated
human	WB	Arterioscler Thromb Vasc Biol; BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.; Authors - Lin Sisi S
mouse	WB,IHC	Int J Mol Sci; Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells; Authors - Kenji Ono
mouse		Sci Rep; Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.; Authors - Ermes Candiello
human	IF	PLoS One; TNF Stimulates Nuclear Export and Secretion of IL-15 by Acting on CRM1 and ARF6.; Authors - Ouyang Suidong S