Anticorps Polyclonal de lapin anti-ARF1

• Phare
• Validé par KD/KO

ARF1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 20226-1-AP

Synonymes

EC:3.6.5.2, ADP-ribosylation factor 1, ADP ribosylation factor 1

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Galerie de données de validation

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  WB analysis using 20226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20226-1-AP

  WB result of ARF1 antibody (20226-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 20226-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20226-1-AP

  WB result of ARF1 antibody (20226-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HeLa cells.

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  WB analysis of HepG2 using 20226-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20226-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20226-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20226-1-AP

  IP result of anti-ARF1 (IP:20226-1-AP, 3ug; Detection:20226-1-AP 1:600) with HeLa cells lysate 2440ug.

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  IHC staining of human breast cancer using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20226-1-AP (ARF1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20226-1-AP (ARF1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20226-1-AP (ARF1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 20226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20226-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 20226-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARF1 (20226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293, cellules HeLa, cellules HepG2, cellules SKOV-3, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral humain, tissu de cancer du côlon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

20226-1-AP cible ARF1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ADP-ribosylation factor 1
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 18-21 kDa
• Numéro d’acquisition GenBank: NM_001658
• Symbole du gène: ARF1
• Identification du gène (NCBI): 375
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.

Publications

Species	Application	Title
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai
human,mouse	WB	Nat Commun; Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation; Authors - Mutian Jia
human	WB	Nat Commun; ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling; Authors - Maximilian Hirschenberger
human	WB	PLoS Pathog; Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.; Authors - Tao Wang
human	WB,IF	J Cell Sci; The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.; Authors - Yuanyuan Su
human	WB	Sci Rep; In vivo monitoring of the recruitment and activation of AP-1 by Arf1.; Authors - Etienne Sauvageau

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cristian (Verified Customer) (04-26-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 20226-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

ARF1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:20226-1-AP)