Anticorps Polyclonal de lapin anti-APPL1

• Phare
• Validé par KD/KO

APPL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

N° de cat : 19885-1-AP

Synonymes

DIP13alpha, DIP13A, Dip13 alpha, DCC-interacting protein 13-alpha, APPL

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Galerie de données de validation

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  WB analysis of human brain using 19885-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19885-1-AP

  WB result of APPL1 antibody (19885-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

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  WB analysis of HT-1080 using 19885-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19885-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 19885-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19885-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19885-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19885-1-AP (APPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19885-1-AP

  IP result of anti-APPL1 (IP:19885-1-AP, 4ug; Detection:19885-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human breast cancer using 19885-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 19885-1-AP (APPL1 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 19885-1-AP

  Immunofluorescent analysis of HepG2 cells, using APPL1 antibody 19885-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 19885-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (19885-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HepG2 using 19885-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (19885-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293, cellules HeLa, cellules HT-1080, tissu cardiaque humain, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19885-1-AP cible APPL1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APPL1 Protéine recombinante Ag13703
• Nom complet: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
• Masse moléculaire calculée: 709 aa, 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC028599
• Symbole du gène: APPL1
• Identification du gène (NCBI): 26060
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].