Anticorps Polyclonal de lapin anti-Apolipoprotein AI

• Phare
• Validé par KD/KO

Apolipoprotein AI Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (1)

N° de cat : 14427-1-AP

Synonymes

APOA1, apolipoprotein A I, ApoA-I, Apo-AI, ApoAI

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Galerie de données de validation

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  WB analysis of human plasma using 14427-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ileum using 14427-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14427-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse liver using 14427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 14427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 14427-1-PBS

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 14427-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 14427-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Apolipoprotein AI (14427-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, plasma humain, tissu cérébral humain, tissu d'iléon humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Informations sur le produit

14427-1-AP cible Apolipoprotein AI dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Apolipoprotein AI Protéine recombinante Ag5793
• Nom complet: apolipoprotein A-I
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC005380
• Symbole du gène: APOA1
• Identification du gène (NCBI): 335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Publications

Species	Application	Title
	IP	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han
mouse	WB,IP	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	WB	J Control Release; Lipid-mediated protein corona regulation with increased apolipoprotein A-I recruitment for glioma targeting; Authors - Yiwei Zhang
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu
human	WB	Theranostics; Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatment; Authors - Te Bu
		Br J Pharmacol; A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice.; Authors - Sanhu Gou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandre (Verified Customer) (02-28-2019)

Detection of both human and mouse APOA1 protein by westernblotImmunoprecipitation of both human and mouse APOA1

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: WB 1/1000
• Cell Tissue Type: HepG2 / Mouse Liver

Apolipoprotein AI Antibody Western Blot,Immunoprecipitation, validation (WB 1/1000 dilution) in HepG2 / Mouse Liver (Cat no:14427-1-AP)