Anticorps Monoclonal anti-Serum amyloid P component

Serum amyloid P component Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1A1D10

N° de cat : 66084-1-Ig

Synonymes

9.5S alpha 1 glycoprotein, amyloid P component, serum, APCS, PTX2, SAP, Serum amyloid P component

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Galerie de données de validation

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  WB analysis of human kidney using 66084-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 66084-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66084-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66084-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66084-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 66084-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human liver cancer using 66084-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 66084-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver using 66084-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, plasma humain
• Résultats positifs en IHC: tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du foie humain, tissu hépatique humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

66084-1-Ig cible Serum amyloid P component dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Serum amyloid P component Protéine recombinante Ag14957
• Nom complet: amyloid P component, serum
• Masse moléculaire calculée: 223 aa, 25 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC007058
• Symbole du gène: APCS
• Identification du gène (NCBI): 325
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Publications

Species	Application	Title
human	IHC	Clin Chim Acta; Combined analysis of serum SAP and PRSS2 for the differential diagnosis of CD and UC; Authors - Weifeng Wang