Anticorps Polyclonal de lapin anti-AP3S2

AP3S2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 15319-1-AP

Synonymes

AP 3 complex subunit sigma 2, AP 3 complex subunit sigma 3B, AP3S2, AP3S3, Sigma 3B adaptin, Sigma adaptin 3b, sigma3b, Sigma3B adaptin

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Galerie de données de validation

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  WB analysis using 15319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15319-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 15319-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15319-1-AP (AP3S2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15319-1-AP (AP3S2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat, tissu ovarien de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15319-1-AP cible AP3S2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP3S2 Protéine recombinante Ag7565
• Nom complet: adaptor-related protein complex 3, sigma 2 subunit
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC002785
• Symbole du gène: AP3S2
• Identification du gène (NCBI): 10239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AP3S2 is a 22-kDa protein that belongs to the adaptor complexes small subunit family. AP3S2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-3 complex is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2).