Anticorps Polyclonal de lapin anti-Annexin A3

• Phare
• Validé par KD/KO

Annexin A3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 11804-1-AP

Synonymes

35 alpha calcimedin, Annexin 3, annexin A3, Annexin III, ANX3, ANXA3, Lipocortin III, PAP III

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Galerie de données de validation

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  WB analysis of HepG2 using 11804-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11804-1-AP (Annexin A3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 11804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11804-1-AP (Annexin A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11804-1-AP

  IP result of anti-Annexin A3 (IP:11804-1-AP, 4ug; Detection:11804-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu pulmonaire de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

11804-1-AP cible Annexin A3 dans les applications de WB, IP, IF, FC, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Annexin A3 Protéine recombinante Ag2381
• Nom complet: annexin A3
• Masse moléculaire calculée: 322 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000871
• Symbole du gène: Annexin A3
• Identification du gène (NCBI): 306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Publications

Species	Application	Title
mouse	FC	Cancer Immunol Res; ANXA3-rich exosomes derived from tumor-associated macrophages regulate ferroptosis and lymphatic metastasis of laryngeal squamous cell carcinoma; Authors - Licheng Xu
human	IHC	Front Immunol; Upregulated of ANXA3, SORL1, and Neutrophils May Be Key Factors in the Progressionof Ankylosing Spondylitis.; Authors - Jie Jiang
rat	IF	iScience; Deciphering the dynamic niches and regeneration-associated transcriptional program of motoneurons following peripheral nerve injury; Authors - Yu Zhang
mouse	IF	Sci Rep; Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.; Authors - R Sartaj
mouse	IHC	Front Med (Lausanne); An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.; Authors - Songyan Gao
mouse	WB,IHC	J Proteomics; Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.; Authors - Fang Jiao