Anticorps Polyclonal de lapin anti-ANP32A

• Phare
• Validé par KD/KO

ANP32A Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15810-1-AP

Synonymes

ANP32A, C15orf1, I1PP2A, LANP, MAPM, Mapmodulin, PHAP1, PHAPI, PP32

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Galerie de données de validation

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  WB analysis using 15810-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15810-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15810-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15810-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15810-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15810-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15810-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15810-1-AP (ANP32A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15810-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15810-1-AP (ANP32A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 15810-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 15810-1-AP (ANP32A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A2780 using 15810-1-AP

  Immunofluorescent analysis of A2780 cells, using ANP32A antibody 15810-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, cellules HeLa, cellules MCF-7, cellules Raji, tissu cérébral de souris, tissu cérébral humain, tissu de thymus de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules A2780

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below

Informations sur le produit

15810-1-AP cible ANP32A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANP32A Protéine recombinante Ag8546
• Nom complet: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• Masse moléculaire calculée: 249 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC007200
• Symbole du gène: ANP32A
• Identification du gène (NCBI): 8125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

Publications

Species	Application	Title
human,chicken	WB	Sci Adv; Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals; Authors - Lei Na
human	WB	Cancer Cell Int; Super enhancer related gene ANP32B promotes the proliferation of acute myeloid leukemia by enhancing MYC through histone acetylation; Authors - Xiaomei Wan ; KD Validated
human	WB	Arch Virol; The interaction of cellular protein ANP32A with influenza A virus polymerase component PB2 promotes vRNA synthesis.; Authors - Xiuli Wei ; KD Validated