• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-TMEM16A/DOG1

TMEM16A/DOG1 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 12652-1-AP

Synonymes

ANO1, Anoctamin 1, DOG1, ORAOV2, TAOS2, TMEM16A, TMEM16A/DOG1, Transmembrane protein 16A



Applications testées

Résultats positifs en IHCtissu de cancer de l'estomac humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu de cancer du poumon humain,

Dilution recommandée

ApplicationDilution
Immunohistochimie (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

12652-1-AP cible TMEM16A/DOG1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène TMEM16A/DOG1 Protéine recombinante Ag3320
Nom complet anoctamin 1, calcium activated chloride channel
Masse moléculaire calculée986 aa, 114 kDa
Poids moléculaire observé114 kDa
Numéro d’acquisition GenBankBC027590
Symbole du gène TMEM16A
Identification du gène (NCBI) 55107
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANO1 also named as DOG1, ORAOV2, TAOS2 and TMEM16A, acts as a calcium-activated chloride channel. It is required for normal tracheal development. ANO1 (or DOG1) is used as an aid in the identification and diagnosis of gastrointestinal stromal tumors (GIST) within the context of an antibody panel, the patient's clinical history, and other diagnostic tests evaluated by a qualified pathologist. The immunogen is the C-terminal of ANO1. This antibody can recognize isoform1 and isoform2 of human ANO1.

Protocole

Product Specific Protocols
IHC protocol for TMEM16A/DOG1 antibody 12652-1-APDownload protocol
IF protocol for TMEM16A/DOG1 antibody 12652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang
ratIHC

Biomed Pharmacother

Calcium-activated chloride channel is involved in the onset of diarrhea triggered by EGFR tyrosine kinase inhibitor treatment in rats.

Authors - Yumi Harada
mouseIHC

Am J Physiol Gastrointest Liver Physiol

miR-128 participates in the pathogenesis of chronic constipation by regulating the p38α/M-CSF inflammatory signaling pathway.

Authors - Yuntian Hong
ratIF

PLoS One

Anoctamin-1 in the juvenile rat urinary bladder.

Authors - Dominika A Bijos

Cell Death Dis

Dual role of Ca2+-activated Cl- channel transmembrane member 16A in lipopolysaccharide-induced intestinal epithelial barrier dysfunction in vitro.

Authors - Jingru Sui
humanIHC

Front Oncol

Primary extra-gastrointestinal stromal tumor of retroperitoneum: Clinicopathologic characteristics and prognosis of six cases

Authors - Jiaxin Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shihab (Verified Customer) (09-17-2021)

I have used this for immunofluorescent stainings in a certain tissue type (cannot disclose) and despite ANO1 antibodies being notorious for such stainings (we have found only 1 other to work properly), this antibody gave very encouraging results on the first attempt at staining. With further optimisation, we should be able to get a decent staining pattern. Very impressed. I will also be trying this in various other applications. Very promising antibody.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100