Anticorps Polyclonal de lapin anti-TMEM16A/DOG1

• Phare
• Validé par KD/KO

TMEM16A/DOG1 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 12652-1-AP

Synonymes

ANO1, Anoctamin 1, DOG1, ORAOV2, TAOS2, TMEM16A, TMEM16A/DOG1, Transmembrane protein 16A

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Galerie de données de validation

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  IHC staining of human stomach cancer using 12652-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12652-1-AP (TMEM16A/DOG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 12652-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12652-1-AP (TMEM16A/DOG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 12652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using TMEM16A/DOG1 antibody (12652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du poumon humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

12652-1-AP cible TMEM16A/DOG1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM16A/DOG1 Protéine recombinante Ag3320
• Nom complet: anoctamin 1, calcium activated chloride channel
• Masse moléculaire calculée: 986 aa, 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC027590
• Symbole du gène: TMEM16A
• Identification du gène (NCBI): 55107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANO1 also named as DOG1, ORAOV2, TAOS2 and TMEM16A, acts as a calcium-activated chloride channel. It is required for normal tracheal development. ANO1 (or DOG1) is used as an aid in the identification and diagnosis of gastrointestinal stromal tumors (GIST) within the context of an antibody panel, the patient's clinical history, and other diagnostic tests evaluated by a qualified pathologist. The immunogen is the C-terminal of ANO1. This antibody can recognize isoform1 and isoform2 of human ANO1.

Publications

Species	Application	Title
mouse	IF	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
rat	IHC	Biomed Pharmacother; Calcium-activated chloride channel is involved in the onset of diarrhea triggered by EGFR tyrosine kinase inhibitor treatment in rats.; Authors - Yumi Harada
mouse	IHC	Am J Physiol Gastrointest Liver Physiol; miR-128 participates in the pathogenesis of chronic constipation by regulating the p38α/M-CSF inflammatory signaling pathway.; Authors - Yuntian Hong
rat	IF	PLoS One; Anoctamin-1 in the juvenile rat urinary bladder.; Authors - Dominika A Bijos
		Cell Death Dis; Dual role of Ca2+-activated Cl- channel transmembrane member 16A in lipopolysaccharide-induced intestinal epithelial barrier dysfunction in vitro.; Authors - Jingru Sui
human	IHC	Front Oncol; Primary extra-gastrointestinal stromal tumor of retroperitoneum: Clinicopathologic characteristics and prognosis of six cases; Authors - Jiaxin Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shihab (Verified Customer) (09-17-2021)

I have used this for immunofluorescent stainings in a certain tissue type (cannot disclose) and despite ANO1 antibodies being notorious for such stainings (we have found only 1 other to work properly), this antibody gave very encouraging results on the first attempt at staining. With further optimisation, we should be able to get a decent staining pattern. Very impressed. I will also be trying this in various other applications. Very promising antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100