Anticorps Monoclonal anti-ANLN

• Phare
• Validé par KD/KO

ANLN Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1B4A8

N° de cat : 66643-1-Ig

Synonymes

Actin binding protein anillin, anillin, actin binding protein, ANLN, scra, Scraps

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Galerie de données de validation

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  WB analysis using 66643-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66643-1-Ig

  WB result of ANLN antibody (66643-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANLN transfected MCF-7 cells.

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  WB analysis of rat brain using 66643-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66643-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, cellules PC-12, cellules U-251, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

66643-1-Ig cible ANLN dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ANLN Protéine recombinante Ag19294
• Nom complet: anillin, actin binding protein
• Masse moléculaire calculée: 1124 aa, 124 kDa
• Poids moléculaire observé: 110 kDa~120 kDa
• Numéro d’acquisition GenBank: BC034692
• Symbole du gène: ANLN
• Identification du gène (NCBI): 54443
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Anillin, an actin-binding protein, is a conserved cleavage furrow component important for cytokinesis. The human anillin protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. It interactions with at least two other furrow proteins, actin and the septins through its C-terminal PH domain and N-terminal actin-binding domain. In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ingression. Anillin has been found to be overexpressed in tumors and may serve as promising marker for pancreatic carcinoma molecular diagnostics as well as a prognostic indicator for non-small cell lung cancers.

Publications

Species	Application	Title
human	WB	Cell Cycle; CDK1-PLK1/SGOL2/ANLN pathway mediating abnormal cell division in cell cycle may be a critical process in hepatocellular carcinoma.; Authors - Ling Li
human	WB	Clin Exp Pharmacol Physiol; PMID: 32198770; Authors - Min Zhang
human	WB	Front Genet; ANLN and KDR Are Jointly Prognostic of Breast Cancer Survival and Can Be Modulated for Triple Negative Breast Cancer Control.; Authors - Xiaofeng Dai
human	WB	Pathol Res Pract; Vasculogenic mimicry-associated novel gene signature predicted prognosis and response to immunotherapy in lung adenocarcinoma; Authors - Lei Zhang