Anticorps Polyclonal de lapin anti-ANKZF1

ANKZF1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20447-1-AP

Synonymes

ANKZF1, Zinc finger protein 744, ZNF744

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Galerie de données de validation

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  WB analysis of BxPC-3 using 20447-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20447-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20447-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20447-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20447-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20447-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20447-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 20447-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 20447-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules COLO 320, cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

20447-1-AP cible ANKZF1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANKZF1 Protéine recombinante Ag14285
• Nom complet: ankyrin repeat and zinc finger domain containing 1
• Masse moléculaire calculée: 726 aa, 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC000238
• Symbole du gène: ANKZF1
• Identification du gène (NCBI): 55139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human	WB,IF	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	bioRxiv; Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats; Authors - Yi-Ju Tseng