Anticorps Polyclonal de lapin anti-ANKRD2

• Phare
• Validé par KD/KO

ANKRD2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11821-1-AP

Synonymes

ANKRD2, ARPP, hArpp

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 11821-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11821-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11821-1-AP (ANKRD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 11821-1-AP

  IP result of anti-ANKRD2 (IP:11821-1-AP, 4ug; Detection:11821-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human kidney using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11821-1-AP (ANKRD2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11821-1-AP (ANKRD2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 11821-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11821-1-AP (ANKRD2 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 11821-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11821-1-AP (ANKRD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain, tissu cutané humain, tissu de muscle squelettique humain, tissu pulmonaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

11821-1-AP cible ANKRD2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANKRD2 Protéine recombinante Ag2389
• Nom complet: ankyrin repeat domain 2 (stretch responsive muscle)
• Masse moléculaire calculée: 37 aa, 2 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC020817
• Symbole du gène: ANKRD2
• Identification du gène (NCBI): 26287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with Ankrd1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. Ankrd2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus

Publications

Species	Application	Title
human	WB	PLoS BIOLOGY; Integrated transcriptomics and epigenomics reveal chamber-specific and species-specific characteristics of human and mouse hearts; Authors - Junpeng Gao
human	WB,IF	Cancers (Basel); Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells.; Authors - Manuela Piazzi ; KD Validated
mouse	IF	Cell Death Dis; The histone code reader Spin1 controls skeletal muscle development.; Authors - Holger Greschik
mouse	WB	Mol Biol Cell; Ankrd2/ARPP is a novel Akt2 specific substrate and regulates myogenic differentiation upon cellular exposure to H(2)O(2).; Authors - Cenni Vittoria V
mouse	WB	Pharmaceuticals (Basel); Metronomic 5-Fluorouracil Delivery Primes Skeletal Muscle for Myopathy but Does Not Cause Cachexia.; Authors - Dean G Campelj
mouse	WB	J Appl Physiol; Early depression of Ankrd2 and Csrp3 mRNAs in the polyribosomal and whole tissue fractions in skeletal muscle with decreased voluntary running.; Authors - Roberts Michael D MD