Anticorps Polyclonal de lapin anti-ANKRD1

ANKRD1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 11427-1-AP

Synonymes

ALRP, ANKRD1, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, HA1A2, MCARP

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Galerie de données de validation

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  WB analysis using 11427-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11427-1-AP (ANKRD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 11427-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11427-1-AP (ANKRD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IF: See 3 publications below

Informations sur le produit

11427-1-AP cible ANKRD1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANKRD1 Protéine recombinante Ag1970
• Nom complet: ankyrin repeat domain 1 (cardiac muscle)
• Masse moléculaire calculée: 319 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC018667
• Symbole du gène: ANKRD1
• Identification du gène (NCBI): 27063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression. This is a rabbit polyclonal antibody produced by the full-length ANKRD1 of human origin

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Metabolic control of YAP and TAZ by the mevalonate pathway.; Authors - Giovanni Sorrentino
mouse	IF	Nat Commun; YAP1/TAZ drives ependymoma-like tumour formation in mice.; Authors - Noreen Eder
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; Glucocorticoid receptor signalling activates YAP in breast cancer.; Authors - Giovanni Sorrentino
		Hum Mol Genet; Exploiting The CRISPR/CAS9 System to Study Alternative Splicing In Vivo: Application to Titin.; Authors - Karine Charton
mouse	IF	Cell Death Dis; The histone code reader Spin1 controls skeletal muscle development.; Authors - Holger Greschik

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Luigi (Verified Customer) (11-29-2018)

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 5 ug
• Cell Tissue Type: Fibroblast

ANKRD1 Antibody Immunoprecipitation, validation (5 ug dilution) in Fibroblast (Cat no:11427-1-AP)