Anticorps Polyclonal de lapin anti-ANGPTL7

• Phare
• Validé par KD/KO

ANGPTL7 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 10396-1-AP

Synonymes

angiopoietin like 7, Angiopoietin like factor, Angiopoietin like protein 7, Angiopoietin related protein 7, ANGPTL7, AngX, CDT6, RP4 647M16.2

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Galerie de données de validation

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  WB analysis using 10396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10396-1-AP (ANGPTL7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10396-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10396-1-AP (ANGPTL7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 10396-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ANGPTL7 antibody (10396-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10396-1-AP cible ANGPTL7 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANGPTL7 Protéine recombinante Ag0596
• Nom complet: angiopoietin-like 7
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC001881
• Symbole du gène: ANGPTL7
• Identification du gène (NCBI): 10218
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANGPTL7, also named as CDT6, is a member of the angiopoietin-like (ANGPTL) family of proteins, which are important regulators of Angiogenesis. It is a secreted 45-kDa glycoprotein that folds into a homotetramer structure linked by disulfide bonds. ANGPTL7 was originally discovered in the stromal layer of the corne, and moderately expressed in the human trabecular meshwork. The chromosomal location of the ANGPTL7 gene (1p36.22) is within the GLC3B locus (1p36.2-1p36.1) of primary congenital glaucoma, supporting ANGPTL7 as a candidate glaucoma gene. ANGPTL7 could have a pathogenic role in glaucoma, and may serve as a potential therapeutic target.

Publications

Species	Application	Title
human	WB	FASEB J; Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation.; Authors - Tong Xu ; KD Validated
mouse	IF	Osteoarthritis Cartilage; Effects of mechanical stress and deficiency of dihydrotestosterone or 17β-estradiol on Temporomandibular Joint Osteoarthritis in mice.; Authors - T Ootake
human	IHC	Front Mol Biosci; An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.; Authors - Hongpan Zhang
human	WB,IF	Genes Cells; Evidence for a role of angiopoietin-like 7 (ANGPTL7) in extracellular matrix formation of the human trabecular meshwork: implications for glaucoma.; Authors - Comes Núria N
human	WB	Commun Biol; ANGPTL7, a therapeutic target for increased intraocular pressure and glaucoma; Authors - Kavita Praveen
human	WB	Hum Mol Genet; Rare protective variants and glaucoma-relevant cell stressors modulate angiopoietin-like 7 expression; Authors - Inas F Aboobakar

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (01-05-2024)

The antibody worked well for immunofluorescence on frozen mouse eye sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Eye