Anticorps Polyclonal de lapin anti-ANGPTL4

• Phare
• Validé par KD/KO

ANGPTL4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

27

Avis (1)

N° de cat : 18374-1-AP

Synonymes

angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL4, ARP4, FIAF, HFARP, NL2, PGAR, pp1158, PSEC0166, UNQ171/PRO197

---

Galerie de données de validation

• 

  WB analysis using 18374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18374-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18374-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18374-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 18374-1-AP

  IP result of anti-ANGPTL4 (IP:18374-1-AP, 4ug; Detection:18374-1-AP 1:500) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IHC staining of human ovary using 18374-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.

• 

  IF Staining of NIH/3T3 using 18374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HT-29 using 18374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 18374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293, tissu cardiaque de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu de cancer de l'œsophage humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules HEK-293, cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 10 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

18374-1-AP cible ANGPTL4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANGPTL4 Protéine recombinante Ag13237
• Nom complet: angiopoietin-like 4
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC023647
• Symbole du gène: ANGPTL4
• Identification du gène (NCBI): 51129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Publications

Species	Application	Title
		Nat Metab; Resistant starch intake facilitates weight loss in humans by reshaping the gut microbiota; Authors - Huating Li
human	IP	J Clin Invest; Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.; Authors - Akrit Sodhi
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang
mouse	IF	J Pathol; ANGPTL4-αvβ3 integrin interaction counteracts hypoxia-induced vascular permeability by modulating Src signalling downstream of VEGFR2.; Authors - Elisa Gomez Perdiguero
human	IHC	Breast Cancer Res; An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-beta in human breast cancer.; Authors - Misako Sato
human	IHC,WB	J Cell Mol Med; Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure.; Authors - Mingyang Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Niklas (Verified Customer) (11-28-2022)

bright and specific staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:350
• Cell Tissue Type: human fibroblasts